Titusville is a city in and the county seat of Brevard County, Florida, United States. The city's population was 43,761 at the 2010 United States Census. Titusville is on the Indian River, west of Merritt Island and the Kennedy Space Center and south-southwest of the Canaveral National Seashore. It is a principal city of the Palm Bay - Melbourne - Titusville Metropolitan Statistical Area.HistoryThe area was once inhabited by the Ais Indians, who gathered palmetto, cocoplum and seagrape berries. They also fished the Indian River, called the Rio de Ais by Spanish explorers. By 1760, however, the tribe had disappeared due to slave raids, disease and rum. The United States acquired Florida from Spain in 1821. The Seminole Wars delayed settlement.