BOSTER - ELISA and antibody experts PD-1/PD-L1 Pathway in Cancer In normal conditions, mutated antigens from tumor cells will lead to the activation of T cells that bind to and trigger apoptosis in target cancer cells. On the surface of T cells are PD-1 (programmed cell death-1) receptors whose ligands include PD-L1 and PD-L2. Together, PD-1 and PD-L1/PD-L2 are involved with inhibiting T cell response so that the immune response is only initiated when necessary, avoiding chronic autoimmune inflammation. However, tumor cells have utilized the PD-1/PD-L1 pathway to resist anticancer immune responses by producing abnormally high levels of PD-L1. When the PD-L1 ligands bind to the PD-1 receptors on T cells, the T cells become deactivated and anti-tumor activity is obstructed. Learn more here: https://www.bosterbio.com/pathway-maps

Going to AACR in Chicago? Visit SeraCare at booth 3940. We have new products and exciting poster presentations. Join SeraCare and over 20,000 cancer researchers and scientists for the latest cancer research and discoveries at next week's American Association for Cancer Research in Chicago. Visit us at booth 3940 to learn about: Accelerating the implementation of liquid biopsy How our comprehensive NGS QC solution removes doubt and adds confidence Our featured poster presentations on ctDNA, CNV, and fusion RNA QC and validation tools One poster presentation we are particularly excited about is Multi-Laboratory Assessment of a New Reference Material For Quality Assurance of Circulating Tumor DNA Measurements. More details here: https://digital.seracare.com/aacr-2018?utm_campaign=AACR%202018&utm_medium=email&_hsenc=p2ANqtz-93LzvYfsJf0RjWWDSL0ELsF06sYFiHNKomtKKgA94lpNYo9U0DtrzsuG9APPNsNzj6PSHc6X0mLFbLbfeHENgX1JpKQA&_hsmi=61924057&utm_content=61924057&utm_source=hs_email&hsCtaTracking=9b040581-8dd7-45cc-8b46-dfddb177542f%7C312ad491-1b28-4fa8-a57e-a69953b3e88b

iNtRON News: RedSafe, the non-toxic alternative to ethidium bromide, it now on special offer: Buy 2, get 3! RedSafe™ Nucleic Acid Staining Solution (20.000 x) Non-toxic substitute for ethidium bromide Product Number: 21141 Availability: in stock Delivery: 1 day Size: 1 ml (20.000 x concentrated) Price: 98,00 € Use the promotion code RedSafe3for2 - get 3 tubes, pay only for 2 RedSafe™ Nucleic Acid Staining Solution (20,000x) is a safe and economically priced nucleic acid staining solution. It's an alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acids in agarose gels. It emits a green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm. RedSafe™ Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar to using EtBr. The Ames test proves lower mutation with RedSafe™ Nucleic Acid Staining Solution compared to EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Use RedSafe™ Nucleic Acid Staining Solution to be on the safe side and improve the safety of your laboratory! *This offer applies to the list price and cannot be combined with other promotions, quotes or special prices. More information here: http://eshop.intronbio.com/Product/View.asp?pIdx=118&pageno=1&MainItem=L&SubItem=A Webshop here: http://hiss-dx.de/de/redsafetmnucleic-acid-staining-solution-20-000-x.html Or contact us at hiss@hiss-dx.de

AimPlex - Bead based Multiplex Immuno Assays AimPlex Biosciences is pleased announce the release of the Human Th1/Th2/Th17 8-Plex Panel 1. It includes: IFNg, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, TNFa analytes You can find the Product insert here: https://www.aimplexbio.com/human-th-8plex-panel1 The AimPlex Product List can be downloaded at: https://www.dropbox.com/sh/aqmvzul45beh1tw/AAC7zAzFrVka-IskPC4mIoFMa?dl=0 For more information contact us at hiss@hiss-dx.de

BOSTER - Antibody and ELISA experts 3 Main Types of IHC/ICC Fixatives Sample fixation is a required and crucial step for every successful IHC/ICC experiment. Appropriate fixation of samples provides the following benefits during the tissue preparation process: Preserves morphology Prevents tissue degradation Ensures antigenicity Protects cells from damage Choosing which fixing solution to use depends on your sample type and antigen. Since there is no standard fixing solution for all samples, we recommend testing to determine which specific type of solution will be most appropriate and effective for antigen immobilization in your sample. Several fixing solutions are available for use and should be chosen based on the sample type or antigen studied in the experiment. Below are the 3 different categories of fixatives: Aldehyde Fixatives Aldehyde fixatives are di-functional cross-linking agents, which are widely used due to their strong penetrability, low contractibility, and low background. They help keep the cross-linking between tissues and maintain the antigen. Examples include: Formaldehyde/Formalin (most common fixative), Paraformaldehyde, Glutaraldehyde, Bouin’s solution, Zamboni’s solution. What is the difference between formaldehyde, formalin, and paraformaldehyde? Formaldehyde and formalin are often referred to interchangeably. They are similar, but their chemical compositions are in fact different. Formalin (aka NBF) is a saturated water solution consisting of 37 to 40% (w/v) formaldehyde, which is diluted with a phosphate buffer to decelerate polymerization to formaldehyde. As a result, formalin’s fixing ability is decreased. Paraformaldehyde (or polyoxymethylene) is polymerized formaldehyde powder. Dissolving the powder in hot distilled water and adding 10% (v/v) methanol will produce the stabilized formaldehyde solution. Precipitating Fixatives Acetone and alcohol play the role of precipitating sugars and fat in addition to maintaining immunologic competence. Acetone is often chosen for unfixed, snap-frozen tissues and cytological smears due to its strong penetrability and dehydration property. Methanol and ethanol are the most common alcohols selected for cell and tissue fixation because of the similarities of their molecular structures with water, which enables them to substitute water molecules in tissues by competing for protein hydrogen bonds. Non-Aldehyde Fixatives In cases where aldehyde or precipitating fixatives are not optimal choices for the sample type or antigen, non-aldehyde fixatives have been used as alternatives. Examples: Mercuric chloride-based fixatives: These fixatives provide more intense IHC staining while preserving cytological details more effectively than aldehydes. However, this alternative also results in tissue hardening, which may be troublesome. Diimidoester fixation: This fixation method utilizes dimethyl suberimidate (DMS), which offers the advantages of retaining antigen immunoreactivity and eliminates the need to block aldehyde groups. Other non-aldehyde fixatives include carbodiimide, dimethylacetamide, para-benzoquinone, etc. Note: In some situations, these non-aldehyde fixation agents can be mixed with other tissue fixatives for a more optimal solution. For more information visit https://www.bosterbio.com/immunohistochemistry-ihc-technical-resource-center?utm_source=crm:email+newsletter+list&utm_medium=email&utm_campaign=technical+blog&email=ch.schaefer@hiss-dx.de or contact us at hiss@hiss-dx.de

Invivoscribe News: We are excited to announce the completion of our CE-marked IVD LymphoTrack®* Dx Assay suite with the addition of the LymphoTrack Dx TRB Assay. LymphoTrack® Dx TRB Assay on MiSeq® The LymphoTrack Dx TRB Assay - MiSeq® is an in vitro diagnostic product intended to identify and track clonal T-cell receptor (TRB) gene rearrangements using next-generation sequencing (NGS) on the Illumina® MiSeq®. This assay will determine the frequency distribution of TRB Vβ-Jβ gene rearrangements in patients suspected of having lymphoproliferative disease. Testing for both TRG and TRB targets, increases the probability of clonality detection and combination analysis is highly recommended. Furthermore, all our LymphoTrack Dx assays can be multiplexed within a single sequencing run to identify and assess suspected B-cell, T-cell and other lymphoid proliferations of unknown origin. Advantages of Invivoscribe’s LymphoTrack Dx Assays: One-Step PCR Protocol. Generate libraries without separately adding adapters or indices. Multiplex Samples. All LymphoTrack Dx - kits provide multiple indices allowing up to 24 (MiSeq) samples per target to be sequenced at the same time. Multiplex Different Gene Targets PCR products from different Invivoscribe LymphoTrack Dx kits can be pooled into the same library and sequenced on the same flow cell to reduce per sample testing costs. Combining IGHV leader, IGH FR1/2/3, IGK, TRB and TRG LymphoTrack Dx kits allows up to 168 samples to be tested simultaneously on the MiSeq platforms. Unparalleled Sensitivity. NGS methods offer unparalleled clonality detection with the ability to identify the specific sequence of clonal populations. Interpretation and Reporting is made easy with interpretation criteria included in the LymphoTrack Dx Assays Instructions For Use (IFU). The LymphoTrack Dx TRB Assay represents a significant improvement over fragment analysis test methods because in addition to detecting initial clonal populations, it also provides sequence information to track these clonal rearrangements in subsequent samples. LymphoTrack Dx Assays provide a complete solution. Each kit includes reagents, LymphoTrack Dx software and Instructions for Use. The LymphoTrack Dx TRB NGS-based assay provides high sensitivity results with a single multiplex master mix to target conserved V and J regions of the TRB gene locus described in lymphoid malignancies, thereby, reducing sample DNA requirements compared to gel and capillary methods and simplifying the testing workflow. The LymphoTrack Dx TRB master mix primers are designed with Illumina® adapters and 8 (Kit A) or 24 (Panel) different indices. The accompanying LymphoTrack Dx Software is capable of sorting complex NGS data by gene target, offering a second layer of multiplexing. This provides users further opportunity to reduce testing costs per sample by sequencing amplicons from any LymphoTrack Dx MiSeq® Assay (e.g. TRB, TRG, IGH, IGK) at the same time. Tests that detect TRB clonal rearrangements can be useful in the study of B- and T-cell malignancies, as leukemias and lymphomas usually proliferate from a malignant transformation of an individual lymphoid cell. The human TRB gene locus is on chromosome 7 (7q34) and it includes 65 Vβ (variable) gene segments, followed by two separate clusters of genes each containing a Dβ (diversity) gene, several Jβ (joining) genes, and a Cβ (constant) region spread over 685 kilobases. The two Cβ genes, TRBC1 and TRBC2, encode highly homologous products with no functional difference. During lymphoid cell development, the antigen receptor genes undergo somatic gene rearrangements. Specifically, during T-cell development, genes encoding TRB molecules are assembled from multiple polymorphic gene segments that generate Vβ - Dβ - Jβ combinations that are unique in both length and sequence.3 Since leukemias and lymphomas generally share one or more cell-specific or “clonal” antigen receptor gene rearrangement, the LymphoTrack Dx TRB Assay can be used to both identify clonotype sequences and monitor their frequency distribution over time. For more information vist https://www.invivoscribe.com/product/lymphotrack-dx-trb?availabilityTerritory[]=Outside%20North%20America&detection[]=NGS&detection[]=MiSeq%C2%AE&application[]=Clonality& or contact us at ngs@hiss-dx.de

Cell Guidance Systems: Exo-spin™ Wide variety of downstream applications for Exo-spin™ isolated exosomes Downstream applications for Exo-spin™ isolated exosomes: Dynamic Light Scattering (DLS) Electron Microscopy miRNA Isolation and RT-PCR Over-Represented Motif Analysis Small RNA Sequencing Western Blot The number of studies published using Exo-spin™ kits as the chosen method for isolating exosomes is steadily growing. The research presented in these studies ranges across a wide spectrum of exosome-related topics. In this communication, three recent studies are summarized to highlight the variety of downstream applications that can be carried out subsequent to exosome isolation: Vacuole-inducing compounds that disrupt endolysosomal trafficking stimulate production of exosomes by glioblastoma cells. Zehui Li, Nneka E. Mbah, William A. Maltese. (2017) Molecular and Cellular Biochemistry https://link.springer.com/article/10.1007/s11010-017-3130-x?ct=t(Exo_spin11_22_2017)&mc_cid=7bab6f9b13&mc_eid=a11fdcc343 The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Laura Santangelo, Giorgio Giurato, Carla Cicchini, Claudia Montaldo, Carmine Mancone, Roberta Tarallo, Cecilia Battistelli, Tonino Alonzi, Alessandro Weisz, Marco Tripodi. (2016) Cell Reports http://www.cell.com/cell-reports/fulltext/S2211-1247(16)31253-0 Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via Recombinase Polymerase Amplification. Kevin M. Koo, Eugene J.H. Wee, Matt Trau. (2016) Theranostics For more information visit https://www.cellgs.com/items/microvesicles/exo-spinand8482-purification.html or contact us at ngs@hiss-dx.de

SeraCare News: NEW HIV p24 Performance Panel is now immediately available for sale! Material Description: AccuSet™ HIV-1 p24 Performance Panel SAP Part Code: 0800-0362 Lot Number: 10302546 Members 13 Members Total 12 members positive for HIV p24, 1 member negative for HIV Tests include Roche, DiaSorin, Biomerieux, Perkin Elmer, Zeptometrix, Bio-Rad, Avioq, Fujirebio, and Orasure Fill size: 13 x 1.0mL Regulatory Status: Research Use Only (RUO) For more information visit www.seracare.com or contact us at hiss@hiss-dx.de

BOSTER - antibody and ELISA experts Cancer Stem Cell Signaling Pathway Cancer stem cells (CSCs), also known as tumor-initiating cells (TICs), are a subpopulation of cancer cells that possess the self-renewal and multi/pluripotency properties similar to that of normal stem cells. They have been identified to be tumorigenic and linked with inducing metastasis. Several molecular signaling pathways are associated with governing stem cell homeostasis. Normally, these pathways are highly regulated. However, an increasing number of these signaling pathways have been discovered to become dysfunctional in CSCs, which include the JAK/STAT, Hedgehog, Wnt, Notch, NF-κB, PI3K and PTEN signaling pathways. In certain cases, cross-talk between different pathways contribute to CSC population maintenance. Find the poster here: https://www.bosterbio.com/media/pdf/collaterals/pathways/Cancer_Stem_Cell_Signaling_Pathway.pdf?utm_source=crm:email+newsletter+list&utm_medium=email&utm_campaign=pathway+maps&email=ch.schaefer@hiss-dx.de For more information visit www.bosterbio.com or contact us at hiss@hiss-dx.de

EXBIO News: New Antibodies Mouse Monoclonal to HLA-A+B+C+E The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens. Ms anti-HLA-A+B+C+E (clone TP25.99SF) – purified #11-813-C100 Reactivity: Human Application: FC, WB, IHC(F) Field: Immunology Mouse Monoclonal to Lactoferrin The mouse monoclonal antibody LF5-1D2 recognizes lactoferrin, an iron-binding glycoprotein of about 90 kDa, which has anti-inflammatory, immunomodulatory and anti-microbial properties. It also participates in iron homeostasis, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Besides biological fluids it is also found in the secondary granules of neutrophils. Mouse anti-Lactoferrin (clone LF5-1D2) – purified #11-128-C100 Reactivity: Human Application: FC, ELISA Field: Immunology Mouse anti-Lactoferrin (clone LF5-1D2) – PE #1P-128-C100 Reactivity: Human Application: FC Field: Immunology Mouse Monoclonal to TPX2 The mouse monoclonal antibody TPX2-01 recognizes TPX2, a microtubule-associated critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers. In other cases the down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion. Mouse anti-TPX2 (clone TPX2-01) – purified #11-818-C100 Reactivity: Human, Mouse, Rat Application: IP, WB, IHC(P), ICC, ELISA Field: Immunology, Cancer Discontinued Rabbit anti-LIME (polyclonal) – purified Rabbit anti-Csk (polyclonal) – purified KOMBITEST HLA-B27 FITC / HLA-B7 PE For more information visit www.exbio.cz or contact us at hiss@hiss-dx.de

SeraCare News: We just released our NEW Malaria Positive Plasma material! Please read below to learn more details. Material Description: Malaria Positive Plasma SAP Part code: 1 mL = 0325-0083 10 mL = 0325-0084 50mL = 0325-0085 Bulk = 0325-0086 Regulatory Status: Research Use Only (RUO) For more information visit www.seracare.com or contact us at hiss@hiss-dx.de